3D Confocal Microscope Image Enhancement by Richardson-Lucy Deconvolution Algorithm with Total Variation Regularization: Parameters Estimation
نویسندگان
چکیده
منابع مشابه
Richardson-Lucy algorithm with total variation regularization for 3D confocal microscope deconvolution.
Confocal laser scanning microscopy is a powerful and popular technique for 3D imaging of biological specimens. Although confocal microscopy images are much sharper than standard epifluorescence ones, they are still degraded by residual out-of-focus light and by Poisson noise due to photon-limited detection. Several deconvolution methods have been proposed to reduce these degradations, including...
متن کامل3D Microscopy Deconvolution using Richardson-Lucy Algorithm with Total Variation Regularization
Confocal laser scanning microscopy is a powerful and increasingly popular technique for 3D imaging of biological specimens. However the acquired images are degraded by blur from out-of-focus light and Poisson noise due to photon-limited detection. Several deconvolution methods have been proposed to reduce these degradations, including the Richardson-Lucy iterative algorithm, which computes a ma...
متن کاملDeconvolution of Sub-pixeled Richardson-Lucy Algorithm
We introduce a Modiied Richardson-Lucy Algorithm for reducing data from the Subaru AO system. This new algorithm applies Magain's correct sampling approach to the Classical Richardson-Lucy algorithm. We have evaluated its performance for astronomical usage, appearance of the object and photometric accuracy, and compared our results to those obtained with a Classical Richardson-Lucy Algorithm.
متن کاملBlind deconvolution by means of the Richardson-Lucy algorithm
A blind deconvolution algorithm based on the Richardson–Lucy deconvolution algorithm is presented. Its performance in the presence of noise is found to be superior to that of other blind deconvolution algorithms. Results are presented and compared with results obtained from implementation of a Weiner filter blind deconvolution algorithm. The algorithm is developed further to incorporate functio...
متن کاملFluorescence Microscopy Deconvolution Based on Bregman Iteration and Richardson-Lucy Algorithm with TV Regularization
Fluorescence microscopy has become an important tool in biological and medical sciences for imaging thin specimen, even living ones. Due to Out-of-focus blurring and noise the acquired images are degraded and therefore it can be difficult to analyse them. In the last decade many methods have been proposed to restorate these images. One of the most popular methods to restore microscopy images is...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2010
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2009.12.954